Professor George Miloshev, PhD

Head of Laboratory Molecular Genetics
Interests: Genetics and epigenetics
Tel: (+359) 2 979 36 97

Name George Angelov Miloshev
Address Institute of Molecular Biology "Acad. R. Tsanev"
Bulgarian Academy of Sciences (BAS)
Molecular Genetics Lab
“Acad. G. Bonchev” str., bl. 21, lab 523
1113 Sofia, Bulgaria
Telephone (+359) 2 979 36 97
Education and current job:
2018 - present

2004 – 2018

Professor, Laboratory of Yeast Molecular Genetics, Institute of Molecular Biology “Acad. R. Tsanev”, BAS
Associate professor, Head of Laboratory of Yeast Molecular Genetics, Institute of Molecular Biology “Acad. R. Tsanev”, BAS
2004 - 2006 Associate professor, Head of Department of Yeast Genetics, Institute of Molecular Biology “Acad. R. Tsanev”, BAS
1994 - 2004 Assistant professor and group leader with Department of Yeast genetics, at the Institute of Molecular Biology, BAS
1999 - 2000 Visiting scientist at Prof. Sarri Penings’s Lab, Department of Molecular Medicine, Edinburgh University, Edinburgh, UK
1996 - 1997 Visiting scientist at Prof. James Allan’s Lab, Department of Biochemistry, Edinburgh University, Edinburgh, UK
1994 PhD in Molecular Biology
Thesis title: “Histone H1 and the chromatin of yeast Saccharomyces cerevisiae
1991 - 1994 PhD student - Institute of Molecular Biology
1984 - 1991 Research assistant at the Department Chromatin, Institute of Molecular Biology, BAS
1978 - 1983 MSc student in Molecular biology and Genetics, in Department of Genetics, Faculty of Biology, Sofia University “St. Kl. Ohridski”
Project leadership
2017 - 2020NATO Partner country director for NATO SPS MYP Project G5266 
Field detector for genotoxicity by CBRN and explosive devices
2016 - 2019Leader of the molecular genetics part of the project with Bulgarian Science Fund – Molecular mechanisms of immune tolerance - importance for endocrine function of regulatory T-cells
2010 - 2013 Leader of the molecular genetics part of the project with Bulgarian Science Fund – Study of mechanisms by wich Fragile X mental retardation protein 1 regulates neurodevelopment in Drosophila.
Grant number DID 02-35
2010 - 2013 Leader of the molecular genetics part of the project with Bulgarian Science Fund – Study of the effect of environmental pollution on the ionome of natural populations of dandelion for monitoringpurposes.
Grant number DVDU 02/61
2010 - 2013 Leader of the molecular genetics part of the project with Medical University – Plovdiv - YKL-40 – a fundamental and clinical study.
Grant number НО - 1/ 2010
2009 - 2012 Leader of the molecular genetics part of the project with University of Forestry, Sofia – Analysis of DNA damage in indicator plant species from threatened with pollution regions.
Grant number 39/13.04.2009
1995 - 1996 Research Project with Bulgarian Science Fund – Signals used for polyadenylation in the histone mRNAs of Saccharomyces cerevisiae.
Project participation

2005 – 2006 Research project at the Bulgarian Science Fund – Optimization of a novel test for detection of DNA damaging agents in food;
Grant number: MU - B-1512/05, “Young Scientists’ Call”, Section Biology
2004 – 2006 Research project at the Bulgarian Science Fund - Molecular markers in plant taxonomy – application of the RAPD method in the taxonomic study of Galanthus L. in Bulgaria;
Grant number: MU - B -1407/04, Section Biology
Teaching experience

2008 – present Lectures and practicals of  MSc degree Molecular Biology students in the summer semester courses “Apoptosis as a norm and in pathology” at the Department of Cytology, Faculty of Biology, Sofia University
2007 Lectures and practicals for MSc degree students at Plovdiv University “P. Hilendarski” on “Epigenetics and apoptosis”
2004 – present Lectures and practicals of BSc and MSc degree Molecular Biology students in the summer semester courses “Cellular Regulatory Mechanisms” at the Department of Cytology, Faculty of Biology, Sofia University
Professional memberships

1994 – present Union of Scientists in Bulgaria
1994 – present FEBS constituent society
2002 – present Мember of The Finance and Policy Committee of ICYGMB - a yeast molecular biology organization (Bulgarian representative)
Recent original publications
2016Staneva, D., Georgieva, M. and Miloshev, G. 2016 Klyuveromices lactis harbours a functional linker histone encoding gene FEMS Yeast Res, Volume 16 (4), April 2016, Pages 182-192
2016Vasileva, B., Georgieva, M., Staneva, D., Zagorchev, P. and Miloshev, G. 2016 Chromatin modulates cellular response to UV light during the process of chronological ageing International Comptes Rendus de l'Academie Bulgare de Sciences , Volume 69 (12), Pages1592-1602
2016Georgieva, M., Staneva, D. and Miloshev, G. Epigenetic significance of chromatin during cellular ageing and organismal lifespan In D. Hollar and D. Vasudevan (Eds.) Epigenetics, the environment and children's health across lifespans, New York, Springer, pp 21-66, ISBN: 978-3-319- 25323-7.
2015 Georgieva, M., Staneva, D., Uzunova, K., Efremov, T., Balashev, K., Harata, M. Miloshev, G. The linker histone in Saccharomyces cerevisiae interacts with actin-related protein 4 and both regulate chromatin structure and cellular morphology International Journal of Biochemistry and Cell Biology, Volume 59, February 2015, Pages 182-192
2014 Kazakova, M.H., Staneva, D.N., Koev, I.G., Staikov, D.G., Mateva, N., Timonov, P.T., Miloshev, G.A., Sarafian, V.S. Protein and mRNA levels of YKL-40 in high-grade glioma Folia Biologica, Volume 60, Issue 6, 2014, Pages 261-267
2014 Miloshev, G.A., Peycheva, E.N., Dodoff, N.I., Kushev, D.N., Lalia-Kantouri, M. Effect of light transition metal complexes of methanesulfonic acid hydrazide on the viability of yeast Saccharomyces cerevisiae Molecular and Cellular Probes, Volume 28, Issue 5-6, 1 October 2014, Pages 259-263
2014 Ekaterina Peycheva, Radostina Alexandrova, George Miloshev Application of the yeast comet assay in testing of food additives for genotoxicity LWT - Food Science and Technology, Volume 59, Issue 1, November 2014, Pages 510-517
2013 Setchanova, L., Kostyanev, T., Markovska, R., Miloshev, G., Mitov, I.G.  2013. Serotypes, antimicrobial susceptibility, and beta-lactam resistance mechanisms of clinical Haemophilus influenzae isolates from Bulgaria in a pre-vaccination period. Scandinavian J. Infect . Diseases. 45, 2013, 81-87
2012 Staneva, D., Peycheva, E., Georgieva, M., Efremov, T. and  Miloshev, G. 2012. Application of comet assay for the assessment of DNA damage caused by chemical genotoxins in the dairy yeast Kluyveromyces lactis. Antonie van Leeuwenhoek. 08/2012; DOI:10.1007/s10482-012-9793-0
2012 Alexandrova, R.I., Zhivkova, T., Alexandrov, M., Miloshev, G., Georgieva, M., Pantcheva I.,Mitewa, M. 2012.  Cytostatic and cytotoxic properties of Monensic acid and its biometal(II) complexes against human tumor/ non-tumor cell lines. Central European Journal of Chemistry (in press)
2012 Peycheva, E., Tashev, A., Vasileva, Y., Staneva, D., Georgieva, M., Miloshev, G. 2012. Genome rearrangements in the common dandelion Taraxacum officinale web. collected from sites polluted with heavy metals. Comptes Rendus de l’Academie Bulgare, des Sciences, 65 (6): 807 – 814
2012 Georgieva M., Miloshev G. 2012. The linker histone and chromatin of yeast Saccharomyces cerevisiae. In: Histones: Class, Structureand Function (Chang-Hui Shen, ed.), Nova Science Publishers, NY, USA (2011) pp. 59-75, ISBN: 978-1-62100-274-1
2012 George Miloshev, Ekaterina Peycheva, Milena Georgieva. 2012. A sensitive method detects DNA damages caused by food additives. Journal of Bulgarian Academy of Sciences, vol. 1, 10-14., ISSN 0007-3989
2011 Georgieva M., Staneva D., Uzunova K. and Miloshev G. 2011. The deletion of the gene for the linker histone in arp 4 mutant yeast cells is not deleterious. Biotechnology and Biotechnological Equipment, 26 134 – 139
2011 Georgieva M., Roguev A., Balashev K., Zlatanova J., Miloshev G. 2011. Hho1p, the linker histone of Saccharomyces cerevisiae, is important for the proper chromatin organization in vivo. Biophys. Biochem.Acta Gen. Reg. Mechanisms, 1819 (5): 366-374
2011 Peycheva, E. , Ivanova, E. , Ivanov, S., Miloshev, G. (2011) Improved procedure for comet assay on active photosynthetic cells from pea plants. Oxidation Communications, 34 (2), pp. 320-325
2010 M. Draganov, G. Miloshev, N. Popov, M. Murdjeva, D. Staneva, M. Kostova, D. Draganov and N. Tomlekova. 2010. Molecular and cytogenetic criteria for identification of serum-free cell cultures. Biotechnology & Biotechnological Equipment 24(2) Special Edition, pp. 139-143. ISSN 1310-2818
2009 Miteva, R., Georgieva, M., Peycheva, E., Efremov, T., Miloshev, G. 2009. Development of conditions for comet assay application in forensic investigation of rape and other sexual assaults. Biotechnology and Biotechnological Equipment 23 (1), pp. 1093-1094
2009 Peycheva, E., Georgieva, M., Miloshev, G. 2009. Comparison between alkaline and neutral variants of yeast comet assay. Biotechnology and Biotechnological Equipment 23 (1), pp. 1090-1092
2009 Georgieva, M., Efremov, T., Alexandrova, R. and Miloshev, G. 2009. Comet Assay discriminates levels of chromatin compaction, Comptes Rendus de L'Academie Bulgare des Sciences 62 (4), pp. 479-484
2008 Georgieva, M., Harata, M. and Miloshev, G. The nuclearactin-related protein Act3p/Arp4 influences yeast cell shape and bulk chromatin organization, J Cellular Biochem, 2008 May 1;104(1): 59-67
2008 D. Staneva, D. Uccelletti, P. Venkov, G. Milolshev, C. Palleschi. (2008) The Kluyveromyces lactis CPY Homologous Genes – Cloning and Characterization of the KlPCL1 Gene. Folia Microbiol. 53 (4), 325–332
2008 Draganova-Filipova, M., Georgieva, M., Peycheva, E., Miloshev, G., Sarafian, V. and Peychev, P. 2008. Effects of propolis and CAPE on proliferation and apoptosis of McCoy-Plovdiv cell line. Folia Medica 1: 53-59
2007 Alexandrova, R., Vacheva, A., Georgieva, M., Miloshev, G., Mosoarca, E.M., Tudose, R. and Costisor, O. 2007. Investigations on cytotoxic and antiproliferative effects in vitro of a newly synthesized mixed ligand copper (II) complex, Acta Morphologica et Anthropologica, 12: 72-78
2007 Lesseva, M., Christova, I., Miloshev, G. 2007 Cloning and expression of recombinant flagellar protein flaB from Borrelia burgdorferi. Folia medica, 49 (3-4), pp. 58-62